Simultaneous determination of nortriptyline hydrochloride and fluphenazine hydrochloride in microgra

A novel method for the simultaneous high-performance liquid chromatographic determi- nation of nortriptyline hydrochloride and fluphenazine hydrochloride was developed and validated. Fhivastatin sodium was used as internal standard. The determination was performed on a Hypersil Gold Cs column (250 mm × 4.6 mm i.d., 5 μm particle size) at 25 ℃; the mobile phase, consisting of a mixture of formic acid (0.1 M, pH 2.16)-methanol (33:67, v/v), was delivered at a fow rate of 1.1 mL/min and detector wavelength at 251 rim. The retention time of nortriptyline, fluphenazine and fluvastatin was found to be 5.11, 8.05 and 11.38 min, respectively. Linearity ranges were 5.0-1350.0 and 10.0-1350.0 μg/ mL with limit of detection values of 0.72 and 0.31μg/mL, for nortriptyline and fluphenazine, respectively. Results of assay and recovery studies were statistically evaluated for its accuracy and precision. Correlation coefficients (r2) of the regression equations were greater than 0.999 in all cases. According to the validation results, the proposed method was found to be specific, accurate, precise and could be applied to the simultaneous quantitative analysis of nortriptyline and fluphenazine.