Inhibitory effect of voglibose and gymnemic acid on maltose absorption in vivo

AIM To determine whether diabetic care can be improved by combination of voglibose and gymnemic acid ( GA ), we compared the combinative and individual effects of voglibose and GA on maltose absorption in small intestine.METHODS The small intestine 30 cm long from 2 cm caudal ward Treitz's ligament of Wistar rat was used as an in situ loop, which was randomly perfused in recircular mode with maltose (10 mmol/L) with or without different dosages of voglibose and/or GA for an hour. To compare the time course, perfusion of 10 mmol/L maltose was repeated four times.Each time continued for 1 hour and separated by 30 minutes rinse. In the first time, lower dosages of GA (0.5g/ L) and/ or voglibose (2μmol/L) were contained except control.RESULTS Absorptive rate of maltose was the lowest in combinative group (P< 0.05,="" anova),for="" example,="" the="" inhibition="" rate="" was="" about="" 37%during="" the="" first="" hour="" when="" 0.5g/l="" ga="" and="" 2μmol/l="" voglibose="" with="" 10mmol/l="" maltose="" were="" perfused="" in="" the="" loop.="" the="" onset="" time="" was="" shortened="" to="" 30="" minutes="" and="" the="" effective="" duration="" was="" prolonged="" to="" 4="" hours="" with="" the="" combination;="" therefore="" the="" total="" amount="" of="" maltose="" absorption="" during="" the="" effective="" duration="" was="" inhibited="" more="" significantly="" than="" that="" in="" the="" individual="" administration="" (p＜0.05,="" u="" test="" of="" mann-whitney).="" the="" effect="" of="" ga="" on="" absorptive="" barriers="" of="" the="" intestine="" played="" an="" important="" role="" in="" the="" combinative="" effects.conclusion="" there="" are="" augmented="" effects="" of="" voglibose="" and="" ga.="" the="" management="" of="" diabetes="" mellitus="" can="" be="" improved="" by="" employing="" the="" combination.="">