Isolation and phylogenetic analysis of heterotrophic nitrobacteria

A method about the isolation of heterotrophic nitrosebactria and the characteristics of heterotrophic nitrosobaetria were studied, it can be seen from the fluorescence in situ hybridization results of the bio-mem-brahe sample from the bio-ceramic reactor, the spots of green nitrosobacteria are obviously more than those of orange nitrohaeteria. Two heterotrophic nitrohacteria were isolated from the bio-ceramic reactor. By sequencing 16SrDNA and establishing the phylogenic tree, they were identified physiologically and biochemically as Pseudomonas sp.. After 12 d, the COD removal efficiency of wgy55 and wgy68 were 45.03% and 50.85% , the NH4-N removal efficiency of them were 80.12% and 85.93%, and the TN removal efficiency of them were 69.71% and 64.7%. The final concentration of NO2-N of wgy55 and wgy68 was 0.753 mg/L and 0.601 mg/ L, and that of NO3-N was 3.21 mg/L and 3.38 mg/L. These results show that wgy55 and wgy68 have the ca-pability of nitrification and they are heterotrophic nitrobacteria.