Establishment and Application of a Set of Material System for Studying the Resistance to Blast in Ri

The study used Waixuan35, a blast resistant rice material adopted widely previously in South China, Yuexiangzhan, a rice variety with the largest planting area in Guangdong Province at present and with the characteristics of horizontal blast resistance and high harvest index, and Qisizhan, the earliest good eating quality variety in Guangdong Province as parent materials, and created an important material systems for studying agricultural characters such as rice blast resistance genes, their resistance mechanisms and quality by hybridization offspring single grain transfer method and DNA hybridization method, namely recombination inbred line RIL, extend recombination inbred line ERIL and transferred-mutant recombination inbred line TRIL assemblages. In RIL assemblage, various materials of resistance gene combinations for identifying rice blast pathogen strains had been identified from the material system, providing direct material evidence for gene to gene theory. Using the same control and same method, in ERIL assemblage, it was proved that there were several main resistance genes and several minor resistance genes in horizontal resistant Yuexiangzhan,providing preliminary evidences for multigene control in horizontal resistance. A set of stable material assemblage for studying future horizontal resistance had been created. A mutation material of Yuexiangzhan from horizontal resistance to vertical resistance was obtained by wild rice external DNA electric stimulation introduction, and variation in mutation assemblage was certified by SSLP. These studies and materials provided important new materials for studying rice blast resistance mechanisms, gene cloning, resistance genes especially horizontal resistance.