Synthesis of N-Phosphoryl Branched Peptides

H-phosphonates were conveniently prepared by direct transesterification of diphenyl phosphite (DPP) with the corresponding alcohols, without further purification they were reacted with branched peptide methyl ester (L-Leu2-L-LysOMe) through Atherton-Todd method, a series of different substituted alkyloxy (N-phosphoryl-L-Leu)2-L-LysOMe were synthesized, and their structures were confirmed by 31p NMR, ESI-MS, 1H NMR, 13C NMR, IR and elemental analysis.The approach possesses the advantages of easy operation, high yield and inexpensive phosphorylating reagent.