Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wa

A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves.The morphology,cultural characteristics,phospholipid fatty acids,physiological and antagonistic fungus activities of this strain were investigated.A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria.The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa;this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens,such as Botrytis cinema,Cladosporium cucumerinum,Fusarium spp.The isolate EBL-06 can grow well using monosodium glutamate wastewater(MGW)and potato wastewater(PW)as culture medium.The maximum yield of 6.5×109 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28℃,pH 6.0-7.5 using the mixture of MGW and PW(1:9).