All-optical Quantitative Framework for Bioluminescence Tomography with Non-contact Measurement

In this contribution,we present an all-optical quantitative framework for bioluminescence tomography with non-contact measurement.The framework is comprised of four indispensable steps:extraction of the geometrical structures of the subject,light flux reconstruction on arbitrary surface,calibration and quantification of the surface light flux and internal bioluminescence reconstruction.In particular,the geometrical structures are retrieved using a completely optical method and captured under identical viewing conditions with the bioluminescent images.As a result,the proposed framework avoids the utilization of computed tomography or magnetic resonance imaging to provide the geometrical structures.On the basis of experimental measurements,we evaluate the performance of the proposed all-optical quantitative framework using a mouse shaped phantom.Preliminary result reveals the potential and feasibility of the proposed framework for bioluminescence tomography.