Partial Purification and Properties of an Acid Phosphatase from Pearl Oyster Pinctada Fucata

Acid phosphatases (ACPs) are marker enzymes for the detection of lysosomes in cell fractions.However, ACPs in sea creatures are less studied than those on land.An acid phosphatase was partially purified from pearl oyster Pinctada fucata by chromatography on Sephadex G-150 and Con A-Sepharose 4B.The specific activity was 1719 U*mg-1 and with optimum pH (5.0) and temperature (60℃).The enzyme was strongly inhibited competitively by product analog WO3-4 and MoO3-4, but less inhibited by product analog AsO3-4.The enzyme could also be strongly inhibited by heavy metal ions, such as Ag+ and Cu2+, but was not affected by Pb2+.High concentrations of ethanol (64%) and NaF (10-3 mol·L-1) could inhibit the enzyme while low concentration of NaF (