聚乙二醇渗透处理对马尾松种子活力的影响

μU24fμU 1EUATWOAOOμ↔°6↔NSNs+"Vol. 24 No.12000Ae 1 0AJournal of Nanjing Forestry UniversityJan. 2000940001 'V4Eo 1. |A1qOAi T2EE00x0>TAμA∞°Ti0+-/02 Ao 09f"ATYWAOOμ" 6NSE_ AOxE0^0e> -x3NS09 AT4O 2100372008 0曰ND%zEx00OIO E PEG£Eo i,' |ArqAiT2EEOOxO>TA;μA0°Ti i&i~ 1yoy%>EONeEelWeE$bE/YAE2> 1--0x0Aija"作91"|A1E+%axe°"q0A112EE00x0>个A|μA0°1i6 ,N;36AExiyN |AixeTjEOAPEG xTYN- |Aixe2T H2O2 !AiAiT2EE00x0E-c10q0A112EE0OxO> TA0D1000"i iEONe>1 -c10E-PEG Eoi,' |AiA0ia, BAI T2EE00x0μAi 1 1u01Aa9|AOA! if10v0°E 30U0010 ”&"PEGf0Eo 1. ' AiE>TAp0_ EyEAiT2EEOOx00D14-0Aa9A S722.1TA1x+∈EqAe ATAOAtaA 1000 - 2006 2000201 - 0038 - 03Effects of PEG Pretreatment on Seed Vigor of Masson PineYu Fangyuan Liu Yuanf"College of Forest Resources and Environment Nanjing Forestry University Nanjing 210037E@Abstract The effects of PEG pretreatment on seed vigor of masson pinfe Pinus masomicnaf@rere studied. The resultsshowed that seed vigor of masson pine had a significant difference among different pretreatment groups of PEG molecu-lar weighf£oncentration and treating time. The best PEG pretreatment group has been determined. Seed vigor can be .further enhanced by PEG+ H2O2 pretreatment and cold resistance can be strengthened by PEG pretreatment.Key words PEC fOsmoconditioningEV igor indexESeed of masson pinePEG EC000NuAB -0x0EoN1 )Ai21973 Ae Heydecker Ex”TA00A PEG”|Ar00x0&mnjμAAE36Ago0EeμADS10j20 EANT 80 Ae u Woodstock 600 -c10 PEG 34B0D21AT1>-OOxOAuEEia ,B0Ox0>TA! x+0AiE EP6E→PEG Eo i,' |AiOOxOμA -/2- uAu/AE1a-9ND4i2 10;0AiAic°101u0AOUAOx+ 1r00x0° |AiμAND4&t μAzTTa2qOAOA%O0xOμAND%&>qazq0A12EE0xOμAND%i>1个V4Q+^ "uAj[x+0B%1 PEG Eo1.' |ArqOAiTeEEOOxO>TA{μA0°Ti xAEzTETEeμAND%zif12AAT0e -/2-1.1 2AATEONeEuOAAiT2EE00x0-0+00U 1995 i4996jt997 Ae2Ex0/-1+ jQ@2>0j£OMF- PEG6 000 1μET°E1aA+->~1o3ξEG2UEPEG10 000 6i 1@4> TNSEOyA≈g0FEO1" VhoiU-0x°f@H2O2 14土94@> ~NεEONA3ξEu2UjE1.2f"1f@ONeEel4FE 002> ir PEG -0x0Ai ja>y -0Ey91°|A1E+Yax=个@0°Aii2EE00xO>TA{μuA 3.0EOEAi0E0-0 3, ,oEWE)hE为“ -0x0Ag" PEG6 000£PEG10 000fPEC中国煤化工i20% i60%@6!ATE士V组~1 dic3 dic5 dR@E° o)y)>H1/>obxe*9EWE242/yF 9。σx:MYHCNMHGf" 2f0EG /4b00 ,WYEONE∈EeVAEAO>x∈∞TAaOE 40 mL uA PEG EU0°E%0U PEGE0, ,aEOEU 1999-04-08 DP1BEOEU 1999- 11-23.uO>x∞BW0%he$θ: -x60=EADEAT$BA0Oμ' oNSE- AOxE0~0e》 -3is0城1EU;Eja 38万亩数据2000Ae x0μU 85 EU0+ -:_6ue20001) "VEoI," |ArqOAIi2EEOOxO>TA;μA0°IiEOO90DOAEOTA18~ 10 ~ 20 C f(:%bo0A"CEjetWajeOx0Az心°" 0y)> EeVFEOQCO6%oDjf/zp001y3iOD2%- 32%A°eE00°∞006%46 PEG EODμAN6FEo°→Ai i/)b0%aEoO6EGOxOOACaE@.- ,3aT. [E%4 f00000x00ACaE@/b>24 h&θO>ημA0e PEG /bp00laμ+9→E@AεμA7OOONuE.iEE" 3LDEG+ H2O2 /4p00 i"1y0y/}> EONeSOAN; 3δxT%N PEG /4p00xe9126A Ax∈°T + H2O2 E0O°W4p00E-H2O2 uAla>y -0Ey1a 1% i&%2[ 3% EyoO;l/p00962a9T" -cNiAEiecNi0. ,Ey2[>TA}0_Eyiff"4EDEG + μ1A"!Ai 00x%-0/l}>EONeEuμAμA PEG xT%Nxe9T' Ar9620A 3 C TAμ1MA!Ai 2 dEzaT"-CN;AEjecN;0。Ey9T>TA!0, EyjE00T°%- PEG”|Afμ< 1-Nuμ1TA- |AiμA00x0x+9000jff 5teaT" -cN;2a"°" GB2772 - 81TAOA%0x0Vi Ne -/6-~ i HAO@CONkoDDj2OU-cN;2aq" μAI←EtEθE0i3VAE -cN;00x0EyE-cN;2aT"%kaEoEtZ2a~0xAc3qE%EEa -cNzO Ey91>TA:0, Ey2°-cNj0. ,Ey GI= E" }fd£fO .E%6ODC2l 00A ' 20@EOFtμAEOEyEn a0:。≈EOμAOy3f -cN;AEy>TA}0_Ey VI=LSXGIE%6ODfLS fEI84uAc3e" cmf国6I日.CN;0, EyiE2 /ha 102.1 2, i-|ArqOATeEEOOxO-cNzAE9f>TAqμAO°1i1ry0yh2》EeWEEONeSμAuμ/2》f-xe2T |ArRAEEOOxOμA -cNAEiecN;0. Ey9[>TA10. EyE%a10%40+1 1if0ti 1 D;00;*2622> i-xe≌° PEGEoi,' |Ai [1 2>1- PEG -OxAiI&TERFAIEfNaxeTqOqOAi T2EE00x0μA -cN;AE9f>TA|μA0°T12η0i0 0jf .ATieEEOOx0-cN;AE9f>TAjμAO°TixOμACejoEC%- PEG EoL,' |AI9OAITEEOOxOμA-C Table 1 Efes of difret PEG prereatment on seed vigorN;AE9[>TA}0D/%T 63iqEia, βi&μ<020ODμ10σUσμAand germination percent of masson pine”!Ai xe°TE&cxe°[°ATs 721 9 uAAk ,δ |Arxe9Tif xeTA .o.0lawy.0Ey% .0.Ey0A2> 1--0x0Ai iday -0Ey9Tq|AiE+VaμAOyl>> EONe600010W6a10 -0t05s/E1-0> -0x0Acjφ-O> ia>y -0Ey91F→O》26 000206.486 56.736E+VaμA>TA|0。Eey0@e iE%EEI/AU>TA:O ,Eyif3075.680 38.1480EE仫须>个A|0。Ey iEOEPEG _0x0Aε↑a 10410 000176.683 51.23867.50573.303000jta>y -0Ey 20% i$!AE+\a 1 d μuAOu”|Ar00x0166.843 55.553>TA10U。+OEO0D1≌x个”6[6aE>0y)z> EONeODA> 6D0a20 00001.625 12.2160》- |Ai xe9T2u<。u94YOylz>EONe0- AiZzEOOTT2E-0.497.528 67.724253.272 19 .765x0Ai 10 000i day -0Ey 20% i肚a 1 d μA- |Aixe9T00-~CaE@/p0020655.364 45. 344xT0DAa0ia, ,BAi 1iEE00x0μA>TA! E@E统6CxTYNxe°Tipq00yl%》EONeEyAYx+ .2个.01682pAtF 2μAva10i2 ti2 PEG !Af -oxoxiany -0Ey ;exaEyoEoqO~0ti 2 6的;E0Oε“36E0U -0x0Ai iA" Ee 1EYaEyOEO>TA:0. EyO°μA_/2个-0160DE+|AiA"WEμAta》- 1000x0>060100o0ifTable 2 The variation analysis of vigor index of2.2 PEG + H2O2 "A1qOATPEEOOxO>TA|μAO°idifferent PEG pretreatment。jg%2YOy/>> EONela10SNj0A -0x0Ai 10 000A"作ta0iA0* Ae27E>-/2e「 xOOE4-%u-EFa20% iC!AiE土%a 1 d μAxe°7E-00402> 1-A-qEμA-0xOAi1 079.09539.5456.09f0.10=9.0A-qE1 617.03808.5159.13'H2O2 EUO°%p00 |Aijf/ka10-C10EPEG 40A"qETe465.01232. 5032.621%91 2%μA H2O2 Vp00ZmTA10_ Ey -0+01日72.214 EOe177.2088. 6002f 77. 7012%66A PEG "!AiμA00x0>TA10。Ey1B_x0e3 338.3362.249f9%o 9o0Q 6ACaE@%b0OqQA>TA| 0。EyO》0Dx$中国煤化工45.344ifla+E0@TA2PEG40A-怕T日1%i 2%μA H2O2 )MYHCNMHGBjEaOD HO2 A"eE2%μA~ |AfDS1qx79AE*TA}0, .EytE%60A PEG ' |Aila ,B 25%fEGaE甸”|Aila .B 71% iSμ< PEG 4A"E3%μA H2O2 /p00EmAp0. .Ey0>OD 33. 258Ep1001000ifip39邑AT3OAOOμ↔°6NSNs+°μ024%F μ01FU2.3 PEGEoi."!A1q02, 1-AT>- EIATEEOOxO-cNzAE9f>TA;juAO°0A-0x0A; 10 000ja,y -0Ey 20% ic|AiEta 1 d μAxTOAxeT° |A12> i-AI>- EBE)yμAAI TeEE0x0f%a10V0+f 3jf01 3 0D;E00;'36EFEG Eo i.”|Ai02> 1ATti3 PEG EoI, ^|Aq0e, i-AT-E@EAITeEE .>- EOE/EAiTzEE00x0μA -CN;AEOD2>> 1-3iqEμAla ,BE-00x0 -cNεAEe[*TAjμA0°Tiμ< la_ ,BμAOμ2T0i%T°6jEqO>TAμ0_ ,EyO20D/T 6μAO°Table 3Effects of PEG pretreatment on seed vigor1i60AI>-aiqEO/ETEPEG "!Ai μAx+0AOzA+10j& .of different aged masson pine10001997 Ae00x0>TA!0, ,Eyla ,B 37%£1996 Ae00x0>介AT=-EWEI%^|Ar-CNiAE%.0.Ey1a.B%Aj0. Eeyla。β 53%£+995 Ae00x0>TA!0. Eeyla。β1997 Ae≥E00 E(8+*-100020 3545.344057 % iEE1AT> 00x0>TA}0.Eyla B-u作,0 62TEG62.2493411.6%if1996 Ae2E00 E创Wp” 100020 3129 .442PEC3144 .98052.4 PEG 0o"!Aiq0AiTeEEOOx0;1 ToofAa210°fi1995 Ae2E0OE“100000 3023.677 .AiT2EEOOx0Aa%bp Tu01EtE-cNzAE91>TA|A+701997 Ae2E00E:”000PEG37.0601Av°"1000cN;AE 21%2-cN;0.印y 1. 985EmTA!0。f"EE1=A1-204.4222022.624412Ey 23.6842国p<%- PEG”|Ai96E→cNεAE9i>TAIE0ts30Taμ土E@FS° PEG + Aa%pS≈cNεA∈33% E-cN;0, Ey 5.142EmTA}0, eEy 44.631f殿3 .01o0e i0A0f" 1fDEG Eo i,' |AiqOAi2EE00x0-cN;AE9[>TA}ODA- T0μA U2o x+0AEzEO0Eδμz0c38Aci δeAqμAAiuAifμ<2> i--0x0Ai jtay -0Eyi”|AiE+%Aaxe°TμA”|AT%a102> 1-zθDμAx∈°TE60A2>Ec1006 ti 1£@E0O -ENi0nA PEG μA -0x0Aieay -0Ey9i |AiEt%a2qO PEG Eo 1,' |AiμAOyE- E1 6A2%B0D0o00a0a j&oy/}》EONeμAWha10+1A=E-0x0Ai 10 000iay -0Ey 20% j士/a 1 d uA- |Aixe*TDS10xWNjf -/2个2 -016μA/6a10+1A$° ti 2公国-EyOoEOODE(!AA"IE1O-cNzAE9T> TA!μA0°i xT1e700of6CNj0n° |Aixe°TμA1/N4uEa0Uiff" 2ADEG + HO2 AnO0A1EEOOx0qμA>TA}0100o∞°TijSaOD”|AIA- TExiT10/NUEfl2O2 laxy -0Ey0O2%个e xT/NjEPEG + H2O2 "!Aia,BOOxO>TAHμA0-0o;EAUEC |Ai1y3i0DfH2O2 0> -)6AaeTe00x0ia1@AE3axauAN6EoEA1O> -VAxAEOμ6AETO0q%x +0AE∞OOx0AE -中iAE_ 0DA0μA16/pjfμ< EC10 H2O2 A"作1y. B0000°niuμ:OOxOAE -c1y3iOD UD>> [%9μAμ+j0μEx+0AE-" To2>Au0U00xOμAAE -c2θa02D1EC 3%' A"TEDS10Q2>)/NμAεEA00-00iff£"32@> 1UECxOE>AT>-》1 ECE 1oAI>- μA00x0EAT>- 白国E%0丝,BEPEG Eo i,”|Ai la. β00x0>TA|μAx→0AO%k6jf0aEμA+ PEG Eoi,' |A12>)6AU -AO1000oiEU1uE@ToOiAEAAE∈ESSoCOOONODEDEEμAT。°iA0DO》9T"uADP。' x+0AjEf"4EDEG Eo i,' |AiA0OC;Ai TeEE0Ox0μA;1 Aae!A0A! i&jEAOμA0_0δEC PEG 0n' |Ai AtaAE_ EOr00x0AxAx0-μATTAix 1-E%EUAEOOx0μ介ATu01E+Oo;iEUTuE@Oi3EμAA∞EE9 |&+0AaAE00x0AU9-1r0EμAA-ESif21jWTA Ix£0 1 £10%TAafθf1ax旦. 00x0>TA! . +土40EAENS3δ°eEcf+991202 £NOL1a>+Di+9%AAE1E06>O. PEG0y -ci 0x0uADS10.02TπτN$+° *E+985238 :328329 ~ 333£03fEI q1EAo>OEU. PEG Eo i,' |A12a000xOμANDNi. TA!@i;1@:YHCNMHGf~0δETta/- 0EciNXxEOa 40万亩数据