tRNA的应用前景

EuAuuA,NS200620%3阳Ua103lAOA±a°A￡001362000030103-03tRNA HA00Ac°43AA￡-00EBA￡I6μA±}￡”0D1NSOE| MEuli NSNE%Eu000× OEu lINSOOHaEμNeEE|E20001910和0′ EEARNA AaEEITERNA-8AEU IErRNA % euvaHoo0E1a0B40Aa°AQ522+.1AI×± eEASf月RNA EC1AATq480a1AUμAD1·0×0E%3Eau, IADOADE6°aE0 HAuoeII E2·020B0oμAμ1° Liel, B141v2a1l i￡ L DIHA tRNA ha I HAva10a02 E4AE RNA 22a11pA,B正11b1-0360EE00sE00aa0010±aoEa00a1 i EDi3Elai-HA-0×0a￥x÷0AEs A AYDyha1μAM2i·0￡-0± OApAU T》9·E"I"j￡AUA6”-ERNA≠A0÷0a1A0Ec1ד0。°±1.19oNAE士0p°° HA tRNA AaEEIi0uEa2cB-0°±Ea210lu°° xOEEul9 I3Eifnd Ii Eae xAEEA啊00RNA,6001”°±·cI0￡6D0 DOAEAa tRNA 22a1100÷μ AAae eal0oD0900E61000如b004°RNAOeE2106 EaEuEulT1jAUieEaexAEEAC啊0RNAE00×0Ta￥x: OAHAETEEEIEf660啊°a1191 AUEIEHAt0￥yAN-E00Ee°t￡ RNA 913EA,0 Eu tRNA E±p°xAOnDO-ciiEHA tRNA AaEEiiiEf00aDOAaEEIT tRNA OO% DD TAiHAAaEEIrEeVEEOD i02uEuEAuANB% 2n0E34AEEulria, OEj9-06°'iB,I38C士AUμ LAOlAAEOCIIiEBasti Hb“199N9EORaHAAaTHfjaEuAU0-1yaiHAi2E; ia1@AE AE tRNAPhe. AUAe%HA l RNase HA DNA AaEEODAHuA1a94B23CH40AO DC Eu ii %4v4EOHA0H OAjE ITjENDitiA-E9-0DiiiE tRNAPhe Tai-DPEIAi iaz100UDEOy tRNA HANDi021aHooxOE 1n EUO EauA DNa AaEE Irou iaia% iimslia土UAE1 aA eHAOHOAC°°i2D6￡020BND%±A÷￡ Pi aydy60T0A±1. tRNA AaEETi±a6AECIiE> tRNA AaEE ITVal1 0E 631, E%4uEu3puAAi uAuseYE0o'E. c ii E HADATCHAnyAUi EEg"u RNA iaHADoADug-ieEx tRNA AaEETroDiux-1a iaAUOH0AOCTT20+OEC中国煤化iEμi2E0,a6U￡P911058E÷0AA0AuA0x÷0BoeA"1973iE8A8P8EE0能EDA-06xU: latELEAUO0中的 AajfSchimmel HEE194194EuAF91880D%￡“1993a36￡ ACAa tRNA°±x0Ea0EUw￥ARNAOD1UifNS0 0%Ei ERGEL ifDj aydycra°±6￡uA00D00EAE啊00:0Uo 2U x+ DO AU Ae x@"operational code EoFiei EuAuuA-N2006203阳U土ENeE0194A6020AEμ NeOlA=AE0aOo子￡10。N%·NE土NA;￡aapT°-0aEa90-y10E、A0A￥uAx正EO°土Tp0o土 UAaAExa,"DS AEHA IEiafiE00iEU土aEaEU% Di AYDyEU I￡e6°=EA,uAc±E0u°p0aAc°·c01A--¨0÷0a00Ak00Ec°xka100B。0 HAxex°Ta00AE00a00 e cErcla01EA1000kE60Elca01E0土06IA￡-AUA6x0OA啊 AHAOA^wAUAμA♂0xA1a10￡x÷RNA-6 AEU9li￡EUA0OAAE± e74CoDaNAcU2U×÷0A0Aex0E0 UA3DC IμD0,2BEAE0 HA RNAS61A9E49a土aA6uA6A0alAVo"2EOAAE. AUAex000ia, 3iooi D0 1 B uAA- 20, u la lE"iEaNBEA1 EOECxiDIHA tRNADSAE￡Ecc0°±EaμA·AUA·cD,100iDou00AaET￡ yha aNBEA1E006DNA, NS AvAg0AEc0°土EaEU%￥ PandyμA°±E,e￥μAEa96A-1 OuvhtaaeeAiasou°° iaHa mRNA El i￡xcOauAECE HbAaA. uAlaoH× IDi RNA O°aE0世 Vicia" i RNA ORF HAOOHaEt-06I0AaA, xiDi RNA HAo+oE-EYOxHAajE0a02Di OU RNA/DNA 12 120'iEeaE+A-200U mRNA 3'ITE"03AE120-1y3ioD p AaA, B oD, u 1AATHAEo HAaNBEA EO%ho Ee EiciaHA A i 2h0EuEicia01ENB%土A÷￡y·0x0 avAra11E00 Eu ElHADAEueAA￡20up0s mRNA? i mRNA +ai0.0 CAYDYHA2aA2cA4￥E、90 Eaip AeA6AA-10E0AEa°正kk6A-mNAa0auA0 EC LEAUHAEBo llo-c‰0同‰auA2cA6°6 A'iEEUQIHA们:cE“E0004kAa±AE0。000%0-40A9EAAD2El01 A%20 DDE, Nf" EC BIAjcBA“±aD0A100 RNA HA%a101}A0μAa。 EDi a HEfS° laC RNA2脚a0a00··“A012AeEc°A￡yi￡μ ECEE6Ge6死A486cE0o4102~1036AAc2 CCOEU°TμAlE00×eD0500 PiAYDy2E1E÷‰0,9ND%tRNA6Aa6A0UE、 Ni dAhAO jEOU la laE,ND00av4puAODA1034BjEODERNA-eAEU9174 4E012 Hoox OEHA2p iEADAIC1. 3 tRNA AaEEIix+1 EUliv4AEOuAOH OAf" ylaa-HA mRNA010a°Ep°°x0EEN1?f0 UEuoUo-etrna40BB爬±E0μAa11iE‰NμAAnl±8￡4 SlanUeaey4Y±A÷￡出NApA°±Ea0E2. tRNA 2euv4Hoox OE1 a sE tRNA- mediatedAUAe443EuEE 10+HAH1 L DiEy iEuse protein engineeringf-TRAMPEECEcT In IB AE ia tRNA %BOD idEauAa112400Ui3HAOA1l3E0u0AU20x÷u°×CE0EUACHA6ATcx1Eu0aE6EU10+0Ec0E0%￥0A2000iE°± uEno EUμp0 Eifo tR9·A0A6￥0同4aμ Av4D12c i￡ Leehey HE1E48 3 NA 2euvHAH°°x0Ea3iEf010AA0AEaE0DNOE￥CouA00 EA RNA·0×0a0B0E000EEe4AciE°士 uEaNj onDOL2Ee￥·A0A6￥·0±0N0706啊ypx0 CtRnA、6D0μAND% a trampe ia1EE土ucE0Eaco.0 RNA Dy x20 UTIs EUAEHA AEyaEμAAA0xE“2AAEA1、%￥08 auAv4D72CE=c I0TBAEAuOA TRAMPE Wo DDuAocFEUNDiEcuOUDEa tRNA A12%4Y06aμ lAv4D1CoeEEI°0RNA0 y tRNAjEDEOy tRNA ECO000± a tRACt ly9iA. tRNA Phe 210i‘6-0k1140A±a·AUA6×0A01E土02c0010A6x0们 oeYERNaAaeeITia1AE0。0DA1E0A00D01D0}200E×36D￡0yRNA4±0± Ehitrnauaou lat00÷°|2,EDAa11iE0u20A÷RNA。60 ElaHANDiif0 J TRAMPEAC°EN; JaeAiamou°auAE6‰2E0A们l°0°3EaeA￡ EcEE4ula01E%i0nO,EE中国煤化工EA“坐野directed non-naturalEa605E809i|A、土A67E0E0mIeplacemeinlu xOE A mR-pEE联aE土 uaEx lEECEOEviauA0DE0 x NA HAlO"ip uaEIOyE00140Ax0x960A》￥bSAE|pOE￡al￡五eEμAao%0o￡ea0yAU21uAT°±￡, EOy tRNA A'I^ AO12lyE00ENμuAEA±Ay0kEUE0EOUA0mN-°6E,AUA6x0100uμ°°x0Eμ AloI>Ha26EclNpA-在弟桶E我6面Aa闻E,°Ei fEuAuuA "NS i 2000 Ae 20441 3 EUAC°EOA· CIi E°±xuEa°6￡HNAE035s-4xA0°土a01C-A°土a土eC￡ Ai laE2EOA0EA00N.¨币μA￥00Ec0 Alao HA±ex如Aiaa·0} TuADAEuu'9 xoeEyAi EU°±6￡HNA°TEA°±6￡,- tRNAs108-liaE%B正A0B1ax0191}A2aEA°± ouEafeH12k0 EEOUOA NSDPE了ACc0。0.016DAEu° XOEHA- CAEaD-1E0uA.·“0 EerEaEumuIAjf山UW0c-N°±6E,-2ap0y0NE-c01;20aD--0÷0eEcoE为 <-ClIE,°±xuEa°±￡ Euoea calt”-0ey300U。±￡ HA LyS-tRNA0Cys-R0A-0ONE￥y3框CA9EU0 EaHa trna NA HA Lys o Cys EIA甲EEcEEav EuAGHAAaEE ITE>..+of>"ET EYOxi1yAA1CxiEOAMA oEuiTEO#ecoEqUEl iCav2-lav2lEA、0·00 pOUii HA°±x36 HADAEuH°°×E4B0D0,。6EuE±ec0q"0H‰0Bi9 chiEsA°±0EaE2aE,.0 1a.CIIE°± StEauA26EeE00±00TRAMPE,、6A8T0yAμA0 OAEC%eo”Ay9E01ai2a￡ eu j3HA-AEa0BuA26E6p°×0E410a6AE00a1@p°1E02a为·"、B01aCE,°士Ea2aoxOEna119111 To*a HADAIci+oEC TOOUAC OD2, i EtEAauAOAuaeR, EUE+0AaE10Ai-IDEOf-B20x‰oX-Ea84 s lanueauaaaμ°°xA00DS00A6 Di AiDAEuuooxOES+ AAaE°AE±FRAMPE EC·c0DOAμA1 aBifJohnson X了 A Western012土0AaAE·-06自a0pA0c01a°± p l2Eeμ°°×0E1En0ETE,u°°x2aAEA01311±μAμAx10 DoDY±eci1a°l1akn100UE￡0 y IRNA HA0n2EIE0k°±6HAotuEaUl4iesEY E Turcatti HE 2EOAiaAUT"Ha-C IRNAj3EA, %a110e1 AUHANDiff4tiA-Ee xAiE± uefa.“01a°士Ea3N"7-n- TRAMPE“A-c019e41 :OECOU0Afroben-2-oxa-12-3-ol-4y123- diamino- A0AeμAA6AE±0“ a vo Eu l1v4'4EA1i0propionic acia"NBD-DapEHa20EeWvaEUVdeAEn OUTEJhoEulT2AAToi, uoDDSuAOTfTiOOlTOlAE-26A2 EUlaoDe6U·cpx10,±f"ap0B01·“pA0aEDDEUlauA'i AU2arEtly2aAi21-7I>HaO< 1a°±EaaμA4aA6°Io,00k1 atevCHAFEBeAP1 EUlEY Giege R et e. Curr Opin Stract Bio/Et998E8E288i:1A00币uA.0×0uA% aReuEa1 DAICifEU2EY Basti MM et al. Nat Struc Bio/Ct99623258i44u°×Es-D,6°x°aE=k|μA°自￡U3 Y Meinnel t ef al. J Mol Biot￡1991￡20￡205i°208ex° Ealae u·" OUNi OnDOuODPE1l9 lμA°eex± EU5EY Hipps D et a,EMBO/E+9s+4s:001405Ea土%EU业 AE IPOFE E TRAMPE iE00U10Tcu6 Schimmel p et al, Proc Natl Acad Sci us1939e°Cys I0AT'ua2TEe,心[A山0NB%yEa,0E0e235u°° XOE lOAEE/a111Aa40D0±ouA0AEEU8EY Frugier M et aL. EMBO JCH994ft322218i2226TRAMPE OUEU IT 4 4E00H 0A-12Aa > 0D0 0 EU9fY Henderson BS et al. Bioorg Med ChemEt E5EM071i107900±0uA0p°1a°×0E0013ijt0 ig E010ty Leehey Ma et a,B0mm956235:1629±0EekA1aA6°±0EadE000eu° EDi aDAU11￡12297112302HA aNS DOOEE%0 UODiEAUt OA00OEOi c0122Y Noren C) et al. Science 2+9892344298212188IEouADADiEul i SlamaevHEhs ic>> o1 EU13eY Anthony SJ et al. TIBSEt9902+408001403Ax Eas HA°±9Ea26E003a0k1aE0AuA286atl aaad sci us4￡1994291￡中国煤化工l￡00E00u÷01aE0AcEa1auA2une￡CNMHGnSocEt997EtIs98i°1TRAMPE 1iEOAoUla, BDAEuFxOE14i2aet al. Chem Biolit99724C73917499.0AeμAskE6￡10U0x÷BAup°×EA到EMmY,Jmmx984“2“909A-NE土‰a￡cAy9uco%Aa0-1EU18EY Ohtsuka H et al. Nucleic Acids Symp Serft997E37#1251